ISO 19574:2022 pdf download – Footwear and footwear components — Qualitative test method to assess antifungal activity (growth test). 9 Preparation of test spore suspension 9.1? Apply strains within 4 generations. 9.2? Inoculate the micro-fungi spores onto the MEA medium (7.3) surface or standard PDA medium surface, and incubate at 28 °C ± 2 °C until the surface is full of micro-fungi spores (about 1 week to 2 weeks for Aspergillus sp./Penicillium sp. and 2 weeks to 3 weeks for Trichophyton sp.). 9.3? In each culture tube or plate, place 5 ml of physiological saline (7.4), and a non-toxic wetting...